Study of changes in the activity levels of purine nucleoside phosphorylase and the role of nitric oxide in the development of pathology in alloxan-diabetic rats

The problem of treating diabetes mellitus has not been resolved to this day; it requires a detailed study various links pathogenesis at the molecular level. In diabetes, conditions arise for formation oxidative stress. Diabetes is associated with early development cardiovascular complications, immune e system destroyed. Endothelial cells produce nitric oxide (NO), substance that able maintain balance vascular tone, coagulation and inflammation. Purine nucleoside phosphorylase (PNP) one most important enzymes purine metabolism, which characterizes status organism. aim was enzymatic activity PNP, as well determine levels in blood serum, liver pancreas albino rats simulated alloxan diabetes. results our studies on detection changes PNP model alloxandiabetic showed late stage, completely suppressed, indicates significant body, content increases. Understanding complex metabolic disorders interact NO may provide us additional therapeutic options reduce morbidity mortality mellitus. Indicators can be used paraclinical indicators diagnosis identify clarify degree pathological process, nature course, stage disease, contributes appointment individualized adequate therapy.